Premier pôle européen de recherche, de soins et d’enseignement sur les maladies génétiques créé en 2007 au cœur du campus de l’Hôpital Necker-Enfants malades. Sa mission ? Mieux comprendre les maladies génétiques pour mieux les guérir.
L’Institut Imagine est un endroit unique au monde qui rassemble chercheurs, patients, médecins et personnels de santé dans une architecture créatrice de synergies au service d’une même ambition : changer la vie des familles touchées par les maladies génétiques.
The laboratory "Molecular mechanisms of hematological disorders and therapeutic implications" (team A. Levescot) is looking for an Engineer in Immunology
https://www.institutimagine.org/en/olivier-hermine-and-thiago-trovati-maciel-181
Chronic autoimmune diseases often persist within specific tissues despite systemic immunosuppressive therapy. A major open question is whether this persistence simply reflects sustained immune activation, or whether it arises from genetic and functional evolution of immune clones that progressively alter their behavior and contribute to tissue damage or transformation.
Our research team explores this question in the context of autoimmune enteropathies, in particular refractory celiac disease, conditions where we have shown that tissue‑resident T cells (TRM) can acquire somatic alterations that favor their local persistence and disrupt intestinal homeostasis. These clonally expanded T cells may act as autonomous pathogenic units, sustaining inflammation and, in some cases, driving progression toward lymphoproliferative disorders.
By combining patient‑derived samples, organoid and gut‑on‑chip models, and single‑cell and long‑read multi‑omics approaches, our goal is to connect genetic, cellular, and tissue‑level mechanisms underpinning therapy resistance and continuum between autoimmunity and malignancy
Malamut G, Guégan N, … , Levescot A. Clonal Lymphocyte Expansions and JAK-STAT Pathway Mutations Define a Pathogenic Continuum Driving Resistance to Gluten-Free Diet in Celiac Disease. Gastroenterology. 2026, doi: 10.1053/j.gastro.2026.03.025 ;
Levescot A, Cerf-Bensussan N. Loss of tolerance to dietary proteins: From mouse models to human model diseases. Immunol Rev. 2024, doi: 10.1111/imr.13395 ;
Levescot A, Malamut G, Cerf-Bensussan N. Immunopathogenesis and environmental triggers in coeliac disease. Gut.2022, doi: 10.1136/gutjnl-2021-326257 ;
Cording S et al., Oncogenetic landscape of lymphomagenesis in coeliac disease. Gut. 2022, doi: 10.1136/gutjnl-2020-322935 :
Ettersperger J, et al., Interleukin-15-Dependent T-Cell-like Innate Intraepithelial Lymphocytes Develop in the Intestine and Transform into Lymphomas in Celiac Disease. Immunity. 2016, doi: 10.1016/j.immuni.2016.07.018.
We are seeking a motivated and skilled research engineer to join our translational research team working at the interface of immunology, genetics, and intestinal pathology. The successful candidate will contribute to projects aiming to understand the mechanisms driving immune continuum between in autoimmune disease and lymphoma, particularly in enteropathies. She/he will be primarily responsible for the establishment, maintenance, and characterization of human intestinal primary cell lines and organoid culture. She/he will actively participate in functional studies exploring epithelial-immune cell interactions in close collaboration with wet‑lab biologists and bioinformaticians.
Establish and maintain primary cell cultures intestinal and organoid from patient‑derived biopsies ;
Perform co‑culture experiments of organoids with human immune cells (T cells, intraepithelial lymphocytes) and monitor responses by microscopy, cytometry, and molecular assays ;
Process samples for transcriptomic, proteomic, and imaging analyses ;
Contribute to single‑cell and spatial transcriptomic workflows (sample preparation, QC, archiving) ;
Ensure compliance, traceability, and proper documentation of samples and experiments.
Master’s degree or engineering diploma in cell/molecular biology, biotechnology, or related field ;
Strong experience in mammalian cell culture, flow cytometry, molecular biology ;
Excellent organizational, problem‑solving, and teamwork skills ;
Written and spoken proficiency in English.
Fixed term contract for 12 months, possibility of extension ;
Location: Institut Imagine, Paris, France ;
Salary: based on experience (€ 2868 to € 3070)
Please send your application (CV, motivation letter, and contact information of 1–2 referees)
At Institut Imagine, we encourage a diversity of profiles and career paths. All applications meeting the criteria outlined in the job offer are welcome, in an environment where everyone can work with respect and inclusiveness.
